亚精胺通过抑制NF-κB/NLRP3炎症小体通路减轻LPS诱导的小鼠急性肺损伤

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Abstract Objective The purpose of this study was to observe the effect of spermidine on the expression and activation of NLRP3 in lipopolysaccharide (LPS) - induced acute lung injury (ALI) mice. Methods Effectof spermidine on respiratory function in ALI mice was detected by a small animal ventilator, and the survival rate of mice was detected. Pathological score were used to evaluate the effect of spermidine on the morphological changes of lung tissue in ALI mice, and levels of IL-1β and IL-18 protein levels in bronchoalveolar lavage fluid (BALF) of ALI mice were detectd by ELISA. The qPCR was used to detect the expression of IL-1β, IL-18, NLRP3, ASC, pro-caspase-1 and NF-κB in mouse lungs; IκB expression of mice was detected by Western blots. Results Spermidine could improve the respiratory function and survival rate of ALI mice, alleviate LPS induced lung pathological damage, and reduce IL-1β, IL-18, NLRP3, ASC, pro-caspase-1, NF-κB and IκB expression of ALI mice. Conclusions Spermidine may reduce the assembly and activation of NLRP3 inflammasome by inhibiting the activation of NF-κB in the lung tissue of ALI mice, thereby inhibit the expression of inflammatory factors, and ultimately lessening ALI. This study can provide an experimental basis for exploring the mechanism of ALI from the perspective of regulating endogenous energy metabolism.

Key words： spermidine acute lung injury lipopolysaccharide inflammatory corpuscle

Received: 11 December 2022

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	Articles by authors
	LUO Yutao
	SU Qing
	LI Rui
	YANG Rui
	ZHENG Tongqi
	WEI Yunfu
	SUN Guoying

Cite this article:

LUO Yutao,SU Qing,LI Rui等. Spermidine reduces LPS-induced ALI by inhibiting the NF-κB/ NLRP3 inflammasome pathway[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(1): 6-12.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I1/6

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