白藜芦醇抑制TREM-1表达并减轻脂多糖诱导的小鼠急性肺损伤

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Abstract Objective To observe the effect of resveratrol on the expression of triggering receptor expressed on myeloid cells-1(TREM-1) during acute lung injury (ALI) and the effect of resveratrol on ALI mice. Methods We observe the effect of resveratrol on the survival rate of lipopolysaccharide (LPS) (Mice were treated with a lethal dose of LPS 50 mg/kg) -induced ALI mice; To detect the effect of resveratrol on the respiratory function of ALI mice induced by LPS (10 mg/kg); To observe the effect of resveratrol on the pathological damage, the level of total protein, total cell count, neutrophil count, LDH activity in bronchoalveolar lavage fluid (BALF), and the effect of resveratrol on TREM-1, IL-1β, IL-18 expression and MPO activity in lung tissue of ALI mice. Results We observed that resveratrol can improve the survival rate and respiratory function of LPS induced ALI mice, reduce pathological damage, vascular permeability, inflammatory infiltration, and decrease the expression of inflammatory factors of lung tissue. This study is the first to observe that resveratrol can reduce the expression of TREM-1 mRNA in macrophages of stressed by LPS, and its mechanism may be related to MAPK and NF-κB signal pathway. Conclusion Resveratrol downregulates the expression of TREM-1 in LPS-induced ALI mice through MAPK and NF-κB pathway, and can alleviate ALI.

Key words： acute lung injury resveratrol triggering receptor expressed on myeloid cells-1 lipopolysaccharide

Received: 12 February 2023

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R363

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	Articles by authors
	ZHOU Wei
	XIAO Minglei
	Wei Yunfu
	LIU Sihan
	ZHANG Zhe
	GUO Jia
	DENG Junjie
	ZHU Aiping
	SUN Guoying

Cite this article:

ZHOU Wei,XIAO Minglei,Wei Yunfu等. Resveratrol inhibits TREM-1 expression and alleviates LPS-induced acute lung injury in mice[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(2): 8-15.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I2/8

[1] 张晓慧, 陈聪敏, 丁新耘, 等. 丹参酮ⅡA通过调控中性粒细胞胞外诱捕网保护脓毒症小鼠急性肺损伤[J]. 陆军军医大学学报, 2023, 45(1): 38-44.
[2] 李敏. 重症肺炎患者中性粒细胞/淋巴细胞比值和红细胞分布宽度及其28 d死亡风险的预测价值[J]. 金宝搏官方188学报 (医学版), 2021, 18(4): 255-258.
[3] Morales-Ortiz J, Deal V, Reyes F, et al.Platelet-derived TLT-1 is a prognostic indicator in ALI/ARDS and prevents tissue damage in the lungs in a mouse model[J]. Blood, 2018, 132(23): 2495-2505.
[4] Bouchon A, Dietrich J, Colonna M.Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes[J]. J Immunol, 2000, 164(10): 4991-4995.
[5] Bouchon A, Facchetti F, Weigand MA, et al.TREM-1 amplifies inflammation and is a crucial mediator of septic shock[J]. Nature, 2001, 410(6832): 1103-1107.
[6] Zysset D, Weber B, Rihs S, et al.TREM-1 links dyslipidemia to inflammation and lipid deposition in atherosclerosis[J]. Nat Commun, 2016, 7: 13151.
[7] Kang Z, Zeng J, Zhang T, et al.Hyperglycemia induces NF-κB activation and MCP-1 expression via downregulating GLP-1R expression in rat mesangial cells: inhibition by metformin[J]. Cell Biol Int, 2019, 43(8): 940-953.
[8] He SM, Sun S, Chen AQ, et al.Hypoxia regulates cytokines expression and neutrophils migration by ERK signaling in zebrafish[J]. Fish Shellfish Immunol, 2022, 125: 212-219.
[9] Dong L, Tan CW, Feng PJ, et al.Activation of TREM-1 induces endoplasmic reticulum stress through IRE-1α/XBP-1s pathway in murine macrophages[J]. Mol Immunol, 2021, 135: 294-303.
[10] Lara RC, Franco FN, De Araújo GR, et al.The preventive use of resveratrol increases its antioxidant effect by SIRT1 and subclinical anti-inflammatory action in Neuro-2A cells[J]. In Vitro Cell Dev Biol Anim, 2022, 58(10): 979-986.
[11] Xia EQ, Deng F, Guo YJ, et al.Biological activities of polyphenols from grapes[J]. Int J Mol Sci, 2010, 11(2): 622-646.
[12] Mokrá D.Acute lung injury - from pathophysiology to treatment[J]. Physiol Res, 2020, 69(Suppl 3): S353-S366.
[13] Simão F, Matté A, Pagnussat AS, et al.Resveratrol preconditioning modulates inflammatory response in the rat hippocampus following global cerebral ischemia[J]. Neurochem Int, 2012, 61(5): 659-665.
[14] Hou Y, Zhang Y, Mi Y, et al.A Novel Quinolyl-Substituted Analogue of Resveratrol Inhibits LPS-Induced Inflammatory Responses in Microglial Cells by Blocking the NF-κB/MAPK Signaling Pathways[J]. Mol Nutr Food Res, 2019, 63(20): e1801380.
[15] Jiang H, Duan J, Xu K, et al.Resveratrol protects against asthma-induced airway inflammation and remodeling by inhibiting the HMGB1/TLR4/NF-κB pathway[J]. Exp Ther Med, 2019, 18(1): 459-466.
[16] Fan R, Zhang Y, Botchway BOA, et al.Resveratrol Can Attenuate Astrocyte Activation to Treat Spinal Cord Injury by Inhibiting Inflammatory Responses[J]. Mol Neurobiol, 2021, 58(11): 5799-5813.
[17] Zhao Y, Song W, Wang Z, et al.Resveratrol attenuates testicular apoptosis in type 1 diabetic mice: Role of Akt-mediated Nrf2 activation and p62-dependent Keap1 degradation[J]. Redox Biol, 2018, 14: 609-617.
[18] Thiel G, Rössler OG.Resveratrol regulates gene transcription via activation of stimulus-responsive transcription factors[J]. Pharmacol Res, 2017, 117: 166-176.
[19] Amini P, Nodooshan SJ, Ashrafizadeh M, et al.Resveratrol Induces Apoptosis and Attenuates Proliferation of MCF-7 Cells in Combination with Radiation and Hyperthermia[J]. Curr Mol Med, 2021, 21(2): 142-150.
[20] 粟青, 李文浩, 朱爱萍, 等. 白藜芦醇减轻脂多糖诱导的小鼠急性肺损伤[J]. 基础医学与临床, 2019, 39(08): 1125-1130.
[21] Cheng KT, Xiong S, Ye Z, et al.Caspase-11-mediated endothelial pyroptosis underlies endotoxemia-induced lung injury[J]. J Clin Invest, 2017, 127(11): 4124-4135.
[22] Song L, Zhou F, Cheng L, et al.MicroRNA-34a Suppresses Autophagy in Alveolar Type II Epithelial Cells in Acute Lung Injury by Inhibiting FoxO3 Expression[J]. Inflammation, 2017, 40(3): 927-936.
[23] 杨世勇, 何毅, 宋大强, 等. 基于TLR4/NF-κB途径的白藜芦醇减轻LPS致小鼠急性肺损伤的机制研究[J]. 中国药房, 2020, 31(09): 1034-1039.
[24] 余天会, 李永, 甘波, 等. 白藜芦醇干预变应性哮喘小鼠对血红素加氧酶-1表达、炎性因子及氧化应激的影响[J]. 疑难病杂志, 2018, 17(12): 1375-1379.
[25] Dolinay T, Kim YS, Howrlak J, et al.Inflammasome-regulated cytokines are critical mediators of acute lung injury[J]. Am J Respir Crit Care Med, 2012, 185(11): 1225-1234.
[26] Rong W, Liu C, Li X, et al.Caspase-8 Promotes Pulmonary Hypertension by Activating Macrophage-Associated Inflammation and IL-1β (Interleukin 1β) Production[J]. Arterioscler Thromb Vasc Biol, 2022, 42(5): 613-631.
[27] Aumiler V, Balsara N, Wilhelm J, et al.WNT/β-catenin signaling induces IL-1β expression by alveolar epithelial cells in pulmonary fibrosis[J]. Am J Respir Cell Mol Biol, 2013, 49(1): 96-104.
[28] Bernal-Martinez L, Goncalves SM, De Andres B, et al.TREM1 regulates antifungal immune responses in invasive pulmonary aspergillosis[J]. Virulence, 2021, 12(1): 570-783.
[29] Sun GY, Guan CX, Zhou Y, et al.Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury[J]. Regul Pept, 2011, 167(1): 56-64.
[30] Yuan Z, Syed M, Panchal D, et al.TREM-1-accentuated lung injury via miR-155 is inhibited by LP17 nanomedicine[J]. Am J Physiol Lung Cell Mol Physiol, 2016, 310(5): L426-438.
[31] Dong L, Zhou Y, Zhu ZQ, et al.Soluble Epoxide Hydrolase Inhibitor Suppresses the Expression of Triggering Receptor Expressed on Myeloid Cells-1 by Inhibiting NF-kB Activation in Murine Macrophage[J]. Inflammation, 2017, 40(1): 13-20.
[32] Liu T, Zhou Y, Li P, et al.Blocking triggering receptor expressed on myeloid cells-1 attenuates lipopolysaccharide-induced acute lung injury via inhibiting NLRP3 inflammasome activation[J]. Sci Rep, 2016, 6: 39473.
[33] Gu Y, Deng X, Liang C, et al.Soluble triggering receptor expressed on myeloid cells-1 as a serum biomarker of early neurologic deterioration and prognosis in acute supratentorial intracerebral hemorrhage[J]. Clin Chim Acta, 2021, 523: 290-296.
[34] 朱爱萍. 白藜芦醇对急性肺损伤中TREM-1表达的影响及机制 [D]. 长沙: 金宝搏官方188, 2019.