Mechanism of Icariin I promoting proliferation of mouse spermatogenic stem cells
LIAO Tianlong, CHU Xuetong, YANG Xiaoping
Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, Department of Pharmacy, School of Medicine, Hunan Normal Univercity, Changsha 410013, China
Abstract：Objective The medicine icariin I is applied to mouse spermatogonial stem cells to explore the effect of the medicine on the proliferation function of the cells and the related mechanism for the effect. Methods The changes in the value-added ability of mouse spermatogenic stem cells in the presence of icariin I were detected by using the MTT and EdU cell proliferation methods. The changes of Bmi1, p-Akt, and Akt proteins in mouse spermatogenic stem cells in the presence of icariin I were detected by Western Blot experiment. The expression of related genes was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Finally, inhibitors and siRNA were used to explore regulatory relationship between p-Akt and Bmi1 protein. Results Icariin I at the concentration of 0.5-1µM could significantly enhance the proliferation of mouse spermatogenic stem cells as compared with that in the control group (P<0.05). Meanwhile, within the same concentration range, the expression levels of p-Akt and Bmi1 proteins in mouse spermatogenic stem cells were significantly increased under the action of drugs (P<0.05). P-Akt and Bmi1 protein levels were significantly down-regulated in the AKT inhibitor group, and the difference was statistically significant with the control group (P<0.05). There were no significant changes in Akt mRNA levels in the siBmi1 group compared with the control group (P>0.05). Conclusion Icariin I can enhance the proliferation of mouse spermatogonial stem cells, specifically by activating Akt signaling pathway to up-regulate the expression of Bmi1.
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