Abstract：ObjectiveTo investigate the expression of T helper cell 17(Th17), T regulatory cells (Treg) and related cytokines in patients with chronic periodontitis (CP), and to analyze its clinical significance.MethodsFrom January 2020 to June 2022, 90 CP patients and 45 periodontally healthy subjects were selected from the Department of Stomatology of our hospital. They were divided into control group (n= 45), mild group (n= 45) and moderate to severe group (n=45). The levels of Th17 and Treg in peripheral blood were detected by flow cytometry, and Th17/Treg was calculated. The levels of serum interleukin-6(IL-6), interleukin-17(IL-17), interleukin-10(IL-10), transforming growth factor-β (TGF-β), CC chemokine receptor 6(CCR6) and CC chemokine ligand 20(CCL20) were detected by enzyme-linked immunosorbent assay. The correlation between peripheral blood cells and related cytokines and periodontal clinical indicators were analyzed by Pearson correlation coefficient.ResultsThe peripheral blood Th17 and Th17/Treg in the mild group and the moderate-severe group were higher than those in the control group, and Treg was lower than that in the control group. Th17 and Th17 / Treg in peripheral blood of moderate to severe group were higher than those in mild group, and Treg was lower than that in mild group. There were significant differences in serum cytokine levels among the three groups. The levels of IL-6, IL-17, CCR6 and CCL20 were the highest in the moderate and severe group, followed by the mild group, and the lowest in the control group. The levels of IL-10 and TGF-β were the highest in the mild group, followed by the control group, and the lowest in the moderate and severe group. Pearson correlation analysis showed that Th17, Th17/Treg, IL-6, IL-17, CCR6 and CCL20 were positively correlated with probing depth (PD) and attachment loss (AL). Treg was negatively correlated with PD and AL, while IL-10 and TGF-β were not correlated with PD and AL.ConclusionThe imbalance of Th17/Treg cells in peripheral blood of CP patients is accompanied by abnormal expression of related cytokines. The changes of serum IL-6, IL-17, CCR6 and CCL20 concentrations are closely related to the severity of CP, which may promote the development of CP.
[1] 肖炜卓, 张龙, 王晓冬, 等. 慢性牙周炎患者龈下菌斑宏转录组学分析[J]. 中国医药导报, 2019, 16(21): 21-23. [2] 高丽萍, 毛小荣. Th17和Treg细胞可成为自身免疫性疾病治疗的新靶点[J]. 胃肠病学和肝病学杂志, 2019, 28(3): 341-344. [3] 王萍, 林羽, 李合. Th17/Treg炎性细胞因子对自身免疫性疾病合并肺部感染病情的评估价值[J]. 中华医院感染学杂志, 2022, 32(6): 866-870. [4] Ditoro D, Harbour S N, Bando J K, et al.Insulin-Like Growth Factors Are Key Regulators of T Helper 17 Regulatory T Cell Balance in Autoimmunity[J]. Immunity, 2020, 52(4): 650-667. [5] Thapa B, Pak S, Kwon H J, et al.Decursinol Angelate Ameliorates Dextran Sodium Sulfate-Induced Colitis by Modulating Type 17 Helper T Cell Responses[J]. Biomol Ther, 2020, 28(2): 211. [6] Lee H R, Yoo S J, Kim J, et al.Ab0031 t helper 17 cells were significantly decreased by mitochondrial electron transport chain complex inhibitor in patients with rheumatoid arthritis.[J]. Ann Rheum Dis, 2020, 79(1): 312-318. [7] 傅靖菲, 刘怡. Th17细胞在牙周炎中的表达与应用的研究进展[J]. 北京口腔医学, 2019, 27(6): 347-350. [8] Adriawan I R, Atschekzei F, Dittrich B O, et al.Novel aspects of regulatory T cell dysfunction as a therapeutic target in giant cell arteritis[J]. Ann Rheum Dis, 2022, 81(1): 124-131. [9] Zheng Y, Dong C, Yang J, et al.Exosomal microRNA‐155‐5p from PDLSCs regulated Th17/Treg balance by targeting sirtuin‐1 in chronic periodontitis[J]. J Cell Physiol, 2019, 234(11): 20662-20674. [10] Jokstad A.The 2018 AAP/EFP classification of periodontal diseases, a focus on "risks" as a faux ami and language gone on holiday[J]. Clin Exp Dent Res, 2019, 5(5): 449-451. [11] 孙雯, 徐艳. 淋巴细胞与牙周炎骨免疫调节[J]. 口腔生物医学, 2022, 13(2): 67-72. [12] Moser T, Akgun K, Proschmann U, et al.The role of TH17 cells in multiple sclerosis: Therapeutic implications[J]. Autoimmun Rev, 2020, 19(10): 1026-1028. [13] Kaneko S, Kondo Y, Yokosawa M, et al.The RORt-CCR6-CCL20 axis augments Th17 cells invasion into the synovia of rheumatoid arthritis patients[J]. Mod Rheumatol, 2018, 28(5): 814-825. [14] 赵莉, 杨柯, 李宝坤, 等. CCL20、CCR6和Th17在慢性牙周炎患者外周血中的表达[J]. 北京口腔医学, 2020, 28(5): 262-265. [15] 凌晶, 张友健, 宋雄峰. 自身免疫性肝炎患者血清趋化因子和GP73水平变化及其临床意义研究[J]. 实用肝脏病杂志, 2020, 23(6): 825-828. [16] 杜凌燕, 张明娇, 刘鹏飞, 等. 类风湿关节炎患者血清趋化因子配体20与疾病严重程度的相关性研究[J]. 中华预防医学杂志, 2021, 55(2): 226-232. [17] 张丽瑾, 罗梦珺, 陈涛. CCL20及其受体CCR6在肝细胞肝癌中的作用[J]. 生命的化学, 2021, 41(4): 793-798. [18] 赵思淇, 林江. 慢性牙周炎中免疫细胞作用的研究进展[J]. 医学综述, 2020, 26(22): 4390-4394+4403. [19] Liu S, Liu Z, Shan Z, et al.Skewed Th17/Treg balance during progression and malignant transformation of oral submucous fibrosis[J]. Oral Dis, 2021, 28(8): 2119-2130. [20] Li Q, Shan Q, Sang X, et al.Total Glycosides of Peony Protects Against Inflammatory Bowel Disease by Regulating IL-23/IL-17 Axis and Th17/Treg Balance[J]. Am J Chin med, 2019, 47(1): 177-201.
2023,Vol. 20
