咪达唑仑通过下调circ_0000285表达影响宫颈癌CaSki细胞增殖和凋亡的实验研究

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摘要目的：探究咪达唑仑对宫颈癌CaSki细胞增殖和凋亡的影响及可能机制。方法：用不同剂量（0、15、30、60 μg/mL）咪达唑仑干预CaSki细胞,CCK-8法、流式细胞术分别测定细胞增殖活性和凋亡,蛋白质印迹法测定Ki-67、pro-caspase3、cleaved-caspase3蛋白表达,RT-qPCR法测定circ_0000285表达。通过转染circ_0000285小干扰RNA或过表达载体构建干扰circ_0000285表达或过表达circ_0000285的CaSki细胞,再用0或60 μg/mL咪达唑仑干预,上述相同方法测定细胞增殖活性、凋亡及相关蛋白（Ki-67、pro-caspase3、cleaved-caspase3）表达。结果：CaSki细胞经咪达唑仑干预后,增殖活性、Ki-67、pro-caspase3蛋白表达量降低,细胞凋亡率、cleaved-caspase3蛋白表达量升高,且细胞中circ_0000285表达量降低。干扰circ_0000285表达后,CaSki细胞增殖活性、Ki-67、pro-caspase3蛋白表达量降低,细胞凋亡率、cleaved-caspase3蛋白表达量升高。过表达circ_0000285逆转咪达唑仑对CaSki细胞增殖、凋亡的影响。结论：咪达唑仑可能通过下调circ_0000285抑制宫颈癌CaSki细胞增殖,并促进细胞凋亡。

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	张江川
	吴丹
	蒋伟
	张春梅

关键词：宫颈癌,咪达唑仑,circ_0000285,细胞增殖,凋亡

Abstract：ObjectiveTo explore the effect and possible mechanism of midazolam on the proliferation and apoptosis of cervical cancer CaSki cells.MethodsCaSki cells were intervened with different doses（0,15,30,60 μg/mL）of midazolam,and the cell proliferation activity and apoptosis were measured by CCK-8 method and flow cytometry,respectively. The protein expressions of pro-caspase3 and cleaved-caspase3 were determined by western blotting,and the expression of circ_0000285 was determined by RT-qPCR. After CaSki cells that interfered with circ_0000285 expression or overexpressed circ_0000285 were constructed by transfecting circ_0000285 small interfering RNA or overexpression vector,they were intervened with 0 or 60 μg/mL midazolam,and then the same method sas above were used to measure cell proliferation activity,apoptosis and the expression of related proteins（Ki-67,pro-caspase3,cleaved-caspase3）.ResultsAfter CaSki cells were intervened by midazolam,the proliferation activity and the protein expressions of Ki-67 and pro-caspase3 were decreased,but the apoptosis rate and the protein expressions of cleaved-caspase3 were increased,and the expression of circ_0000285 in the cells was decreased. After interfering the expression of circ_0000285,the proliferation activity and the protein expressions of Ki-67 and pro-caspase3 in CaSki cells were decreased,but the apoptosis rate and the protein expressions of cleaved-caspase3 were increased. Overexpression of circ_0000285 reversed the effects of midazolam on the proliferation and apoptosis of CaSki cells.ConclusionMidazolam may inhibit the proliferation of cervical cancer CaSki cells and promote cell apoptosis by down-regulating circ_0000285.

Key words：cervical cancer midazolam circ_0000285 cell proliferation apoptosis

收稿日期:2022-09-07

中图分类号:	R737.33
	R363

通讯作者:张春梅,E-mail：452335757@qq.com

引用本文:

张江川, 吴丹, 蒋伟, 张春梅. 咪达唑仑通过下调circ_0000285表达影响宫颈癌CaSki细胞增殖和凋亡的实验研究[J]. 金宝搏官方188学报(医学版), 2023, 20(5): 38-43. ZHANG Jiangchuan, WU Dan, JIANG Wei, ZHANG Chunmei. Experimental study on the effect of midazolam on the proliferation and apoptosis of cervical cancer CaSki cells by down-regulating the expression of circ_0000285. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(5): 38-43.

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http://yxb.hunnu.edu.cn/CN/或http://yxb.hunnu.edu.cn/CN/Y2023/V20/I5/38

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