Preparation and Evaluation of Monoclonal Antibody to SOAT1, a New Biomarker for Liver Cancer
1.Department of Medical Laboratory, College of Medicine, Hunan Normal University, Changsha 410013, Hunan, China; 2. Immunodiagnostic Reagents Engineering Research Center of Hunan Province, Hunan Normal University, Changsha 410013, Hunan, China; 3. Lianyuan No.1 Middle School, Loudi 417100, Hunan, China; 4. Clinical Laboratory, Chenzhou Hospital of Traditional Chinese Medicine, Chenzhou 423099, Hunan, China; 5. Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China
Abstract：Liver cancer is a common malignant tumor in clinic. Studies have shown that sterol O-acyltrans-ferase 1 (SOAT1), a key enzyme in cholesterol metabolism, is closely related to the occurrence and develop-ment of liver cancer. Therefore, SOAT1 has great potential as a biomarker for the cancer. Herein, the gene encoding SOAT1 was obtained from the Huh-7 liver cancer cell line. After prokaryotic expression and pro-tein purification, the purified recombinant SOAT1 protein was used as an antigen to immunize mice. A total of 5 anti-SOAT1 monoclonal antibody cell lines, named 1F3, 1G3, 1D6, 2F8 and 4D11, respectively, were obtained by using hybridoma and other technologies. Among them, 4D11 was able to recognize the SOAT1 protein in Huh-7 cells and clinical liver cancer tissue samples. Subsequently, 4D11 was sent to a biological company for sequencing, and the amino acid sequences of the antibody variable regions were obtained. In conclusion, an anti-SOAT1 monoclonal antibody was successfully prepared by recombinant SOAT1 protein, which would not only provide the raw materials for the subsequent application of SOAT1 protein in develo-ping liver cancer companion diagnostic kits, but also lay a foundation for the establishment of SOAT1-based liver cancer diagnostic methods.
曾冰洁, 蒋 情, 张钰萍, 徐 叶, 刘如石, 王庆林, 梁湘辉. 肝癌生物标志物SOAT1单克隆抗体的制备及评估[J]. 生命科学研究, 2023, 27(3): 267-274. ZENG Bingjie, JIANG Qing, ZHANG Yuping, XU Ye, LIU Rushi, WANG Qinglin, LIANG Xianghui. Preparation and Evaluation of Monoclonal Antibody to SOAT1, a New Biomarker for Liver Cancer. Life Science Research, 2023, 27(3): 267-274.
