Abstract：To investigate the expression, clinical significance and functional mechanism of tektin 2 (TEKT2) in ovarian cancer (OV), and provide new ideas in early diagnosis and treatment of OV, multiple databases were combined to explore the functions of TEKT2 in OV. Firstly, the expression of TEKT2 in the OV tissue was analyzed using GEPIA2 database. The prognostic value of TEKT2 in patients with OV was explored by GEPIA2 and TIMER databases and Kaplan-Meier plotter. Secondly, the correlation between TEKT2 and tumor protein 53 (TP53) in OV was explored by Pearson correlation analysis, and the relation between TEKT2 prognostic value and TP53 mutation type was analyzed using Kaplan-Meier plotter. Thirdly, the correlation between TEKT2 and immune cells was analyzed by TIMER database. Finally, the expression of TEKT2 in OV cells was verified by real time-PCR, and the effect of TEKT2 on the proliferation of OV cells was evalua-ted using Cell Counting Kit-8 (CCK-8). The results showed that TEKT2 was highly expressed in OV tissue, which was related to a good prognosis of OV patients, and TEKT2 was an independent prognostic factor. In OV patients, TEKT2 was positively correlated with the expression of TP53, and in wild-type TP53 patients, TEKT2 had prognostic value. High expression of TEKT2 was closely related to macrophages and neutrophils, in which obvious correlation existed between arm-level deletion of TEKT2 and neutrophil infiltration. More importantly, CCK-8 assay showed that TEKT2 could inhibit the proliferation of OV cells. In conclusion, this study found that TEKT2 may affect the prognosis of OV patients by regulating immune cell infiltration and inhibiting OV cell proliferation.
袁美莉, 李 妍, 赵 亮. 多数据库联合探究TEKT2在卵巢癌中的作用[J]. 生命科学研究, 2023, 27(2): 170-178. YUAN Meili, LI Yan, ZHAO Liang. Exploration of the Role of TEKT2 in Ovarian Cancer by Combination of Multiple databases. Life Science Research, 2023, 27(2): 170-178.
