Abstract：MicroRNAs (miRNAs) are a class of small RNA molecules that are involved in numerous cellular processes and play a key role in the growth and development of living organisms. Given the importance and structural specificity of miRNAs, they have profound implications for the prediction and assessment of di-seases. In the rapid development of miRNA detection methods, catalytic hairpin assembly (CHA) is a novel thermostatic nucleic acid amplification technology, with high sensitivity, high specificity, simple and conve-nient operation, and no enzyme catalysis in the reaction process. Therefore, it has great potential in miRNA detection. Herein, the principle of CHA technology, and the CHA-based miRNA detection strategy in target identification, signal amplification and signal output were introduced, and the current challenges and pros-pects of this technology were also discussed, aiming to provide some ideas and reference for related research.
