Abstract：Abstract: In order to investigate the toxic effects of chlorophacinone on zebrafish (Danio rerio) embryos/lar-vae, zebrafish embryos were exposed to 0.20 mg/L, 0.30 mg/L, 0.45 mg/L, and 0.67 mg/L of chlorophacinone solution, respectively, and the survival rate, hatching rate, malformation rate, heart rate, spontaneous move-ments, and length of body were recorded daily. The contents of glutathione (GSH) and malondialdehyde (MDA), activities of catalase (CAT) and acetylcholinesterase (AChE) were measured, and total distance and average speed of movement were recorded. The results showed that the survival rates of zebrafish embryos decreased with increasing exposure concentrations. The 96 h-LC50 and 120 h-LC50 values of chlorophacinone were 0.73 mg/L and 0.61 mg/L, respectively. When the exposure concentration was higher than 0.45 mg/L, the number of spontaneous embryo twitches increased, heart rate slowed down significantly, and the rate of mal-formations including pericardial edema and yolk cyst increased significantly. After 120 h of exposure, CAT activity and MDA content in larvae increased significantly, GSH content declined, and AChE activity was also significantly inhibited. Furthermore, chlorophacinone exposure significantly weakened the behavior of larvae. These results indicated that chlorophacinone exposure would have toxic effects on the early develop-ment of zebrafish embryos/larvae.
引用本文:
吴元钊, 俞逸娴, 徐菀忆, 应剑波, 王斌杰, 王继业, 姚伟宣. 氯鼠酮暴露对斑马鱼胚胎/仔鱼的毒性效应研究[J]. 生命科学研究, 2022, 26(4): 321-328. WU Yuan-zhao, YU Yi-xian, XU Wan-yi, YING Jian-bo, WANG Bin-jie, WANG Ji-ye, YAO Wei-xuan. Study on Toxic Effects of Chlorophacinone Exposure on Zebrafish (Danio rerio) Embryos/Larvae. Life Science Research, 2022, 26(4): 321-328.
