Abstract：Abstract: Drosophila melanogaster, as a model organism of both classical and molecular genetics, has become a powerful model for studying gene regulation of organ development and the occurrence of various di-seases. In order to investigate the expression and localization of Mlp84B, and its interactive proteins in Drosophila, firstly, the Drosophila Mlp84B-GFP fusion sequence containing a promoter was cloned into pUAST expression vector, and the constructed recombinant plasmid was injected into the early fertilized eggs of Drosophila using embryo microinjection technique. Secondly, red-eyed Drosophila was obtained by crossing with W1118 flies, and a single red-eyed fly crossed with a balancer to obtain genetically stable transgenic fruit flies. Lastly, successful construction of the transgenic fly was confirmed by detecting the expression of GFP in vivo and the increased expression level of Mlp84B by qRT-PCR. GFP localization showed that endogenous Mlp84B was expressed in the muscle system, and Mlp84B interacted with Act57B in vivo. The ex-perimental results showed that the UAS-Mlp84B-GFP transgenic Drosophila could be used as a marker line of muscle tissue in studying the formation and homeostasis of muscle system.
引用本文:
姜婷婷, 张佳伟, 刘小玲, 聂宏运, 唐 旻, 佘美华, 曾 群. UAS-Mlp84B-GFP转基因果蝇制备及其表达分析[J]. 生命科学研究, 2022, 26(3): 200-207. JIANG Ting-ting, ZHANG Jia-wei, LIU Xiao-ling, NIE Hong-yun, TANG Min, SHE Mei-hua, ZENG Qun. Construction and Expression Analysis of UAS-Mlp84B-GFP Transgenic Drosophila melanogaster. Life Science Research, 2022, 26(3): 200-207.
