Abstract：Abstract: To explore the potential relationship of osteogenic differentiation and adipogenic differentiation of mesenchymal stem cells, the differentially expressed genes related to osteogenic and adipogenic differentia-tion of mesenchymal stem cells in this study were based on the publication statistics, and functional annota-tion and pathway enrichment analysis of these genes were analyzed by DAVID online software. The analysis revealed 281 GO classifications and 13 KEGG pathways for osteogenic differentiation (P<0.01); 317 GO clas-sifications and 47 KEGG pathways for adipogenic differentiation (P<0.01). Then, the protein-protein interac-tion network was analyzed using STRING database, and the network data from STRING database was analyzed by Cytoscape software, the topological characteristics of each node of the network were calculated according to the network data, and 14 key nodes of osteogenic differentiation and 20 key nodes of adipogenic differen-tiation were selected. After that, comparing the key genes in osteogenic differentiation and adipogenic diffe-rentiation using the Venny online tool, and found that 7 genes (including JUNB, SMAD2, SMAD3, MAPK13, BMP4, SRC, RUNX2) were jointly involved in regulating osteogenic and adipogenic differentiation of me-senchymal stem cells. The key genes for osteogenic differentiation and adipogenic differentiation of mesen-chymal stem cells selected above, which can be used to suggest possible mechanisms of abnormal osteogenic and adipogenic differentiation of mesenchymal stem cells, it also provides possible therapeutic targets and new ideas for the pathogenesis and treatment of clinical diseases related to the balance of bone and fat pro-duction such as osteoporosis.
引用本文:
王碧雯, 骆亚莉, 李 研, 马 玉, 周啸天. 间充质干细胞成骨分化与成脂分化关键基因的生物信息学分析[J]. 生命科学研究, 2021, 25(6): 540-547. WANG Bi-wen, LUO Ya-li, LI Yan, MA Yu, ZHOU Xiao-tian. Bioinformatics Analysis of Key Genes of Osteogenic Differentiation and Adipogenic Differentiation of Mesenchymal Stem Cells. Life Science Research, 2021, 25(6): 540-547.
