Abstract：Abstract: Pei′ai 64S is one of the sterile lines derived from PGMS Nongken 58S, with the largest applica-tion area and the lowest critical temperature to induce sterility. However, its critical temperature is higher than that of thermo-sensitive genic male-sterile line 1S, and is not as safe as 1S for hybrid seed production. To probe into the mechanisms underlying the fertility thermo-sensitivity of Pei′ai 64S and to breed the sterile lines with even lower critical temperature and higher hybrid seed production safety, the near-isogenic lines were developed by crossing Pei′ai 64S as female parent with Fengyuan B, followed by backcrossing the sterile plants of hybrid progeny with Fengyuan B twice. The sterile lines among the obtained near-isogenic lines were used for fertility thermo-sensitivity study. The results showed that, under natural high temperature (daily mean temperature≥28 ℃) conditions, the pollens of 40 Pei′ai 64S-derived near-isogenic sterile lines were completely sterile; however, when treated with 23.5 ℃ for 6 days, the lowest pollen fertility was 0 (p5-1S, p28-1S, p12S), the highest was 87.9% (p1S), and the pollen fertility of Pei′ai 64S was 6.6%. These results in-dicated that, under high temperature conditions, the 40 sterile lines are homozygous sterile lines, but the cri-tical sterility-inducing temperatures between different lines were remarkably different. Therefore, it is possi-ble to breed from them the sterile lines with more stable sterility and safer hybrid seed production than Pei′ai 64S. The whole genomes of Pei′ai 64S and its near-isogenic lines were sequenced, and two sites were found to be associated with pollen sterility. They were preliminarily localized between 19 830.36~20 025 kb (length 194.64 kb) on chromosome 7 and between 22 453.62~22 902.05 kb (length 448.43 kb) on chromosome 9, re-spectively.
引用本文:
王思瑶, 潘晓玲, 刘 敏, 李佳馨, 陈慧玲, 毛丹丹, 戴小军, 梁满中, 陈良碧. 不育系培矮64S衍生的近等基因系的育性感温性及基因初定位研究[J]. 生命科学研究, 2021, 25(6): 479-487. WANG Si-yao, PAN Xiao-ling, LIU Min, LI Jia-xin, CHEN Hui-ling, MAO Dan-dan, DAI Xiao-jun, LIANG Man-zhong, CHEN Liang-bi. Thermal Sensitivity of Pollen Fertility and Preliminary Gene Localization of GMS Line Pei′ai 64S and Its Near-isogenic Lines. Life Science Research, 2021, 25(6): 479-487.
