Abstract：Abstract: To explore the effects of different doses of ionizing radiation and conditioned mediums on genomic instability of bone marrow mesenchymal stem cells (BMMSCs) associated with lung cancer, A549 cells and human BMMSCs were taken as research objects, and a model of the radiation-induced bystander effect was established by X-ray radiation and conditioned medium transfer. Cell colony survival rate, micronucleus rate and the number of foci induced by 53BP1 were detected. ELISA was used to detect the levels of ROS, NO and TGF-β1 in conditioned mediums at different time points. The results showed that cell proliferation in IR group, A549-medium group and BMMSCs-medium group decreased (P<0.05), and that the cell micronucleus rate and the number of 53BP1 foci increased (P<0.05). The most obvious change was observed in IR group, fol-lowed by A549-medium group. The levels of TGF-β1, ROS and NO in A549-medium group were signifi-cantly higher than those in BMMSCs-medium group (P<0.05). The experiments indicated that radiation might induce gene instability effect in non-irradiated BMMSCs through ROS, TGF-β1 and NO in the conditioned medium. This effect was similar to injury caused by direct radiation. A549-medium group had a stronger by-stander injury than BMMSCs-medium group.
引用本文:
张利英, 张苡铭, 周妮娜, 卢志伟, 丁 楠, 华君瑞2, 李洋洋1, 周 婷, 刘 颖, 刘永琦. 电离辐射及不同条件培养基诱发骨髓间充质干细胞基因组不稳定性的比较研究[J]. 生命科学研究, 2021, 25(2): 117-123. ZHANG Li-ying, ZHANG Yi-ming, ZHOU Ni-na, LU Zhi-wei, DING Nan, HUA Jun-rui, LI Yang-yang, ZHOU Ting, LIU Ying, LIU Yong-qi. Comparative Study on Genomic Instability of BMMSCs Induced by Ionizing Radiation and Different Conditioned Mediums. Life Science Research, 2021, 25(2): 117-123.
