Abstract：Abstract: To explore the potential pathogenesis of Alzheimer’s disease (AD), critical pathways and hub genes associated with AD in the entorhinal cortex were selected by bioinformatic analysis. The data related to AD in the entorhinal cortex was downloaded from the Gene Expression Omnibus (GEO). By using R packages sva and limma, batch normalization and differentially expressed genes (DEGs) were analyzed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed by using the plugin of ClueGO in the software of Cytoscape. Protein-protein interaction (PPI) network analysis was per-formed using STRING database, and hub genes were selected by cytoHubba plugin in Cytoscape software. The differential expressions of key genes in different cerebral tissues and entorhinal cortex tissues of AD pa-tients and asymptomatic patients were identified using Mann-Whitney U test. Enrichment analysis indicated that DEGs were mainly enriched in positive regulation of leukocyte cell-cell adhesion, extracellular matrix tissue, corticosteroid reaction and porphyrin and chlorophyll metabolic signaling pathways. The hub genes, like VCAM1, CD44, FOS and SPARC, were identified by PPI analysis. The expressions of VCAM1, CD44, FOS and SPARC in the entorhinal cortex and hippocampus were significantly higher than those in the post-central gyrus, temporal cortex, cerebellum and frontal cortex (P<0.05). And the expressions of hub genes in AD patients were significantly higher than those in asymptomatic AD patients (P<0.01). Based on the bioin-formatic analysis related to the entorhinal cortex with AD, the VCAM1, CD44, FOS and SPARC were found to play critical roles in the progression of AD in the entorhinal cortex. These hub genes may be potential tar-gets for AD early intervention. This study may provide a theoretical basis for exploring pathogenesis of AD in the entorhinal cortex.
引用本文:
邵康梅, 张 凡, 蔡宏斌, 魏海萍, 陈圆圆, 葛朝明. 多个GEO芯片联合分析阿尔茨海默病内嗅皮层的关键基因[J]. 生命科学研究, 2021, 25(1): 80-88. SHAO Kang-mei, ZHANG Fan, CAI Hong-bin, WEI Hai-ping, CHEN Yuan-yuan, GE Zhao-ming. Decoding Hub Genes Related to Alzheimer’s Disease in the Entorhinal Cortex Based on a Comprehensive Analysis of Multiple GEO Chip Databases. Life Science Research, 2021, 25(1): 80-88.
