Abstract：Abstract: The incidence of breast cancer ranks first among female malignancies. Previous studies have shown that enhancers can affect the development of tumors by regulating miRNAs. However, it is unclear what kind of regulatory network is formed between enhancers and miRNAs in breast cancer. In order to explore the regulatory network formed by breast cancer enhancers and miRNAs, 220 pairs of enhancer-miRNA regulatory relationships were identified in 112 invasive breast cancer samples and 104 normal tissue samples, including 220 enhancers and 56 breast cancer dysregulated miRNAs. Survival analysis showed that the expression levels of six miRNAs (hsa-mir-195, hsa-mir-671, hsa-mir-3619, hsa-mir-4664, hsa-mir-5003, hsa-mir-5691) were significantly correlated with the survival of patients with invasive breast cancer. Further GO/KEGG functional analysis showed that the target genes of these six miRNAs were significantly enriched in cancer-related biological processes and signaling pathways. The driver genes and network analysis were performed on target genes of the six miRNAs, and a total of six network key genes were identified. Among them, TP53 and CCNE2 genes were significantly related to the survival of patients with invasive breast cancer. In summary, the results provide a theoretical and methodological basis for exploring the regulatory mechanisms of enhancers and miRNAs in breast cancer.
引用本文:
钱明明, 谭政堂, 王文著, 李昶蓥, 邱正良, 郭志云. 乳腺癌增强子-miRNA调控网络识别与分析[J]. 生命科学研究, 2021, 25(1): 64-69. QIAN Ming-ming, TAN Zheng-tang, WANG Wen-zhu, LI Chang-ying, QIU Zheng-liang, GUO Zhi-yun. Identification and Analysis of Enhancer-miRNA Regulatory Network in Breast Cancer. Life Science Research, 2021, 25(1): 64-69.
