Abstract：Abstract: In order to study the protective mechanism of oligomerized proanthocyanidins (OPC) on skeletal muscle injury in overtraining rats, the rats were randomly assigned to control group (C), overtraining group (OM), OPC treatment combined with overtraining group (OOM). There was no exercise intervention in C group, and the other groups underwent 42-day incremental load treadmill exercise to establish skeletal muscle in-jury model caused by overtraining. During the training period, OOM group was administered intragastrically with OPC once a day (150 mg/kg, 5 mL/kg), and the other groups were given an equal volume of distilled water. The rats were sacrificed immediately after the last training, and the morphology of skeletal muscle was ob-served by HE staining. Immunohistochemistry, enzyme-linked immunosorbent assay, radioimmunoassay and other methods were used to detect relevant biochemical indicators: testosterone (T), corticosterone (Cor), crea-tine kinase (CK) and lactate dehydrogenase (LDH) in serum, p38 mitogen-activated protein kinase (p38 MAPK) and its phosphorylated protein, superoxide dismutase (SOD), malondialdehyde (MDA), 3-nitrotyrosine (3-NT) and 8-hydroxy-2-deoxyguanosine (8-OHdG) in skeletal muscle. The results showed that OPC could improve morphological changes of skeletal muscle caused by overtraining, increase serum T/Cor ratio and SOD activity in skeletal muscle significantly (P<0.01), reduce p38 MAPK/p-p38 MAPK expression in skeletal muscle, serum CK activity (P<0.05), MDA, 3-NT and 8-OHdG contents in skeletal muscle significantly (P<0.05 or P<0.01). The above results indicate that OPC can improve oxidative stress and regulate p38 MAPK signaling pathway-related protein expression, thereby protecting skeletal muscle structure and function in over-training rats.
引用本文:
周海涛, 曹建民, 胡 戈, 牛衍龙, 龚 平, 邵芙蓉, 张子坤, 黄景宣, 董爱霞. 低聚原花青素对过度训练大鼠骨骼肌损伤的保护作用机制[J]. 生命科学研究, 2021, 25(1): 24-30. ZHOU Hai-tao, CAO Jian-min, HU Ge, NIU Yan-long, GONG Ping, SHAO Fu-rong, ZHANG Zi-kun, HUANG Jing-xuan, DONG Ai-xia. Protective Effect of Oligomerized Proanthocyanidins on Skeletal Muscle Injury in Overtraining Rats. Life Science Research, 2021, 25(1): 24-30.
