Abstract：Abstract: In order to investigate the effect of Yangyin Huayu Jiedu Decoction (YYHYJDD)-containing serum on the apoptosis of colon cancer cells, HCT116 cells in the logarithmic growth phase were divided into normal group, blank serum control group and serum groups containing low, medium and high doses of YY-HYJDD (at volume fractions of 5%, 10% and 20%). After the cells were treated for 24 h, 48 h, 72 h and 96 h, their proliferation was detected by MTS kit. Subsequently, the cells treated with the drug-containing serum for 48 h were taken as the study subject, and cell apoptosis was observed by AOEB staining method, Annexin V-FITC/PI double staining was used to detect cell apoptosis rate, and Western-blot was used to de-tect B cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) expressions. The results showed that, compared with the blank serum control group, the serum groups containing low, medium and high doses of YYHYJDD inhibited cell activity after treatment with 48 h, with cell apoptosis appearing and the apoptosis rates significantly increased. The expression of Bcl-2 protein was down-regulated in the high-dose group, and the expression of Bax protein was up-regulated in both the middle-and high-dose groups. The Bcl-2/Bax ratio of the middle- and high-dose groups was significantly reduced. The experimental results preliminarily indicate that YYHYJDD-containing serum can promote the apoptosis of colon cancer HCT116 cells, and the mechanism may be related to its influence on the expression of Bcl-2 and Bax proteins.
引用本文:
朱 静, 严小军, 刘红宁, 叶依殷, 尚广彬. 养阴化瘀解毒方促进结肠癌HCT116细胞凋亡的实验研究[J]. 生命科学研究, 2020, 24(5): 376-381. ZHU Jing, YAN Xiao-jun, LIU Hong-ning, YE Yi-yin, SHANG Guang-bin. Experimental Study of Yangyin Huayu Jiedu Decoction on Promoting Colon Cancer HCT116 Cell Apoptosis. Life Science Research, 2020, 24(5): 376-381.
