Abstract：Abstract: Leptin is a protein which is secreted by adipocyte and plays roles in regulating food intake and energy metabolism. In order to obtain the leptin protein with biological activity, the PCR technology was used to amplify the open reading frame (ORF) sequence of leptin from the pituitary of grass carp. The recombinant plasmid (pGEX4T-1-CiLEP) (containing GST marker) was constructed and transformed into the E. coli BL21 competent cells for leptin expression in grass carp. SDS-PAGE and Western-blot analyses showed that the recombinant fusion leptin (GST marker plus recombinant leptin) was expressed with relative molecu-lar mass of 47 kD in grass carp. In vitro injection experiments showed that compared with injection of GST marker, injection of purified recombinant fusion leptin up-regulated the leptin receptor (Lep-R) gene and the JAK2-STAT3 signaling pathway in grass carp pituitary and intestine. These results enrich the theoretical foundation in understanding the function and regulation mechanism of grass carp leptin gene.
引用本文:
罗文婕, 蒋国民, 赵大芳, 熊 鼎, 瞿符发, 曹申平, 徐文倩, 刘 臻, 李建中, 印遇龙. 草鱼重组瘦蛋白的制备及其对JAK2-STAT3信号通路的作用研究[J]. 生命科学研究, 2020, 24(1): 47-54. LUO Wen-jie, JIANG Guo-min, ZHAO Da-fang, XIONG Ding, QU Fu-fa, CAO Shen-ping, XU Wen-qian, LIU Zhen, LI Jian-zhong, YIN Yu-long. Preparation of Recombinant Leptin from Grass Carp (Ctenopharyngodon idellus) and Its Effect on JAK2-STAT3 Signaling Pathway. Life Science Research, 2020, 24(1): 47-54.
2020,Vol. 24
