Abstract：Abstract: Two transcriptional activation systems developed from CRISPR/Cas9, namely Cas9-p300 and dCas9-VPR, were employed to activate germ cell specific genes, and the differences between them in activation efficiency of target genes were compared. Real-time quantitative PCR results showed these two activation systems can activate the expression of most target genes. Cas9-p300 system activated the BLIMP1 gene more efficiently, while dCas9-VPR system activated NANOS2, DAZL and SOX17 genes more efficiently. At the same time, there was no significant difference between these two systems in activation efficiency of DDX4 and TFAP2C genes. Therefore, the study revealed that the different transcriptional activation systems have different efficiencies in activating germ cell specific genes. This will provide a theoretical basis for study of germ cell specialization by transcription activation system, and shed lights on cell fate determination in other developmental systems.
引用本文:
卢佳豪, 黄亚萍, 罗 芳, 肖亚梅, 赵小阳. 比较两种转录激活系统对生殖相关基因的表达调控[J]. 生命科学研究, 2019, 23(5): 345-351. LU Jia-hao, HUANG Ya-ping, LUO Fang, XIAO Ya-mei, ZHAO Xiao-yang. Comparison of Two Transcriptional Activation Systems in Regulation of Germ Cell Specific Genes. Life Science Research, 2019, 23(5): 345-351.
