Abstract： Abstract: Aptamers are single-stranded RNA or DNA sequences which are selected in vitro from a synthetic random oligonucleotide library using the systematic evolution of ligands by exponential enrichment (SELEX) technology. They can be folded into a specific three-dimensional spatial structure and bind to the target substance with high affinity and specificity. In recent years, nucleic acid aptamers obtained by whole-cell SELEX have great application potential in the disease-related field, especially in recognition of cellular molecules and discovery of biomarkers. However, there are still many problems constraining the development of nucleic acid aptamers. The process of screening whole cells is complicated and difficult, and the selected nucleic acid aptamers have poor performance. Obtaining efficiently nucleic acid aptamers is the key to their application. Herein, the advances of new methods and new strategies of cell-SELEX technology and the ap-plication of aptamers in tumor research were reviewed, which would provide reference for relevant researchers.
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邹梦蝶, 鲁妍兵, 李婉明. 细胞特异性核酸适配体筛选新策略的研究进展[J]. 生命科学研究, 2019, 23(4): 305-309. ZOU Meng-die, LU Yan-bing, LI Wan-ming. Research Advances in New Strategies for Selection of Cell-specific Nucleic Acid Aptamers. Life Science Research, 2019, 23(4): 305-309.
