Abstract： Abstract: In order to construct compatible vectors for Nimble Cloning, a new technology of DNA molecular cloning, the plant vector pCambia1304 was used as a reconstruction target in this study. Five Sfi I restriction sites in the pCambia1304 were removed by base substitution. The stability of the reconstructed vector in Agrobacterium and the expression in plants were analyzed. The results showed that the mutations of 5 Sfi I sites had no negative effect on the stability and expression of the vector. This study provides a compatible vector for the Nimble Cloning system and a template and references for reconstructing other vectors of pCambia series into Nimble Cloning system vectors.
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曾妍静, 沈文涛, 庹德财, 黎小瑛, 言 普, 周 鹏. 适用于Nimble Cloning系统的pCambia载体改造[J]. 生命科学研究, 2019, 23(4): 276-280. ZENG Yan-jing, SHEN Wen-tao, TUO De-cai, LI Xiao-ying, YAN Pu, ZHOU Peng. Reconstruction of pCambia Vector Compatible with Nimble Cloning System. Life Science Research, 2019, 23(4): 276-280.
