Abstract：Abstract: Type 2 diabetes mellitus (T2DM) is driven by defects of cellular function in regulating energy homeostasis, and the dysfunction of tissues or organs composed of these cells. In order to investigate and treat T2DM, researchers have to use animal models to reveal the contributions of different cells, tissues and organs. Mice are widely used for T2DM research, since comparative studies and human disease modelling could be performed with well-characterised mouse strains (in terms of their genotype and phenotype). There are many ways to obtain mouse models in study of T2DM, such as spontaneous mutations that lead to obesity and diabetes, caloric excess, surgical and chemical induction, genetic mutation created by transgenic and gene knockout technologies. There are also other mouse models, including those for understanding interactions between genes and environment, peripheral models generated by CRISPR/Cas9 approaches, and models of diabetic nephropathy. Herein, the methods and techniques of constructing T2DM animal models were discussed to help researchers in understanding and choosing T2DM mouse models.
引用本文:
简 磊, 符策岗, 揭 勇. 2型糖尿病小鼠模型构建的研究进展[J]. 生命科学研究, 2019, 23(3): 237-244. JIAN Lei, FU Ce-gang, JIE Yong. Research on Construction of Mouse Models of Type 2 Diabetes Mellitus. Life Science Research, 2019, 23(3): 237-244.
