Abstract：Abstract: In order to preserve duck genetic resources using frozen semen, Youxian partridge ducks (170 d) raised in cages were used. The vitality and survival time of sperm were tested and the osmotic pressure of its diluent was screened. The semen, whose vitality was above 0.5, was frozen using 6% dimethylacetamide (DMA) as the cryoprotectant. The straws of semen were frozen by fumigating at 2 cm above the liquid nitrogen surface for 7 minutes using a portable, simple and patented technical device. The straws of semen were then immerged into liquid nitrogen for at least 10 min and then were thawed in 40 ℃ water bath for 10 s. The frozen semen, which vitality was above 0.3, was stored and Linwu duck females were inseminated with the semen. The results showed that: 1) In different osmotic pressure groups, the 303 mOsm/kg group of LR diluent had the longest sperm survival time, which showed no significant difference when compared with that of the 273 mOsm/kg group (P>0.05), but significant difference with that of the 215 mOsm/kg group (P<0.05); 2) The average vitality of fresh semen from estrus induction was 0.71, and it was 0.61 from the massage method; 3) The average vitality of the thawed semen from estrus induction was 0.33. The average fertility was 85.93% (the highest batch was up to 93.8%) when the duck was inseminated with thawed semen with the vitality above 0.3. The fertility of control group (fresh semen) was 88.17%. In conclusion, this method will provide technical support for the research and application of poultry in China, such as extending the breeding period of male birds in the field of progeny determination, using semen cryopreservation germplasm resources to make up for the shortage of living species conservation, and so on.
引用本文:
袁景阳, 喻宗岗, 张明军, 李 闯, 姚亚铃, 燕海峰. 攸县麻鸭DMA细管精液冷冻技术研究[J]. 生命科学研究, 2019, 23(2): 115-121. YUAN Jing-yang, YU Zong-gang, ZHANG Ming-jun, LI Chuang, YAO Ya-ling, YAN Hai-feng. Study on Frozen Semen of Youxian Partridge Duck by Straw and DMA. Life Science Research, 2019, 23(2): 115-121.
