Abstract：Abstract: CBX7 regulates the cellular lifespan extension and cancer progression. However, it has proved difficult to obtain the full length CBX7 in sufficient quantities for structural studies. Here the process to recombinantly express the CBX7 functional domain CBX7 (aa 145~227) was described. The target recombinant protein was purified by Ni-NTA affinity chromatography and analyzed by SDS-PAGE and Western-blot. Remaining contaminants were further removed by ion-exchange and size-exclusion chromatography. Through this procedure, milligrams of the CBX7 protein could be available for crystal screen in future studies of CBX7 structure and functions.
引用本文:
胡温温, ALTAF Simair, 吕缜一, 陈 婷, 张云龙, 陆昌瑞. 人源CBX7功能结构域的重组表达与纯化[J]. 生命科学研究, 2019, 23(2): 92-99. HU Wen-wen, ALTAF Simair, L?譈 Zhen-yi, CHEN Ting, ZHANG Yun-long, LU Chang-rui. Recombinant Expression, Purification of a Human CBX7 Functional Domain. Life Science Research, 2019, 23(2): 92-99.
