Abstract：Abstract: The most widely used approach for defining gene function is to reduce or completely disrupt its normal expression. Many gene editing tools are used widely in recent years, including RNAi technology and some increasingly developed technologies with efficient enzymes, such as zinc finger nuclease (ZFN), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system (CRISPR/Cas9). In addition to above technologies, NgAgo is also a new gene editing tool published in 2016. For over a decade, gene editing tools represented by the CRISPR/Cas9 technique have been widely used in gene knockout research based on the cellular level and individual level. Here, the related principles and the latest research progresses of these technologies are summarized, and the principle of CRISPR/Cas9 technology and its latest progress in gene knockout animal models are highlighted.
引用本文:
李 妤, 赵红业, 崔 勇, 魏红江, 李梅章. 基因编辑技术的研究进展[J]. 生命科学研究, 2017, 21(3): 268-274. LI Yu, ZHAO Hong-Ye, CUI Yong, WEI Hong-Jiang, LI Mei-Zhang. Research Advances in Gene Editing Technology. Life Science Research, 2017, 21(3): 268-274.
2017,Vol. 21
