Abstract：Abstract: Leucine-rich repeat (in flightless I) interacting protein-1 (LRRFIP1) was originally identified as a protein that interacts with the leucine-rich repeat (LRR) domain of human flightless-I. Its biological role is unknown, and might be important for actin organization. Recent studies have found that LRRFIP1 can promote the expression of type I interferon in mouse fibroblasts and macrophage cells. Over-expressed LRRFIP1 reduced the levels of virus infection, suggesting that LRRFIP1 may be involved in viral infection-induced innate immune response. Liver, as an important human digestive organ, is vulnerable to hepatitis B virus and hepatitis C virus infection. Here, human LRRFIP1 gene was successfully cloned from hepatoma Huh7 cells via overlap PCR method. It was found that over-expression of LRRFIP1 in Huh7 cells up-regulated the expression of interferon (IFN)-β. The results may provide additional information for the physiological functions of human LRRFIP1.
引用本文:
刘 媛, 王文博, 邹自英, 朱 冰, 胡宗海, 熊 杰. Overlap PCR法克隆人LRRFIP1基因及其在人肝细胞中诱导干扰素的作用研究[J]. 生命科学研究, 2016, 20(6): 492-496. LIU Yuan, WANG Wen-Bo, ZOU Zi-Ying, ZHU Bing, HU Zong-Hai, XIONG Jie. LRRFIP1 cDNA Clone Through Overlap PCR and Its Role in the Induction of Interferon in Human Hepatocytes. Life Science Research, 2016, 20(6): 492-496.
2016,Vol. 20
