Abstract：Abstract: To investigate the offspring sex ratio of Tibetan antelope and determine whether sex-biased selection was existent, 116 Tibetan antelope placentas from Zhuonai Lake lambing region in Kekexili Nature Reserve and 32 placentas from Tuzi Lake lambing region in Arjin Mountain National Nature Reserve were collected, which was based on the reproductive characteristics. Then three pairs of bovine sex-specific primers were selected based upon references, and DNA-based sexing was used to determine the sex chromosome type of the placenta samples, by which the sex of the newborns and the sex ratio among them were identified. The results showed that there were 76 females and 72 males among the total 148 placenta samples. The offspring sex ratio was 1︰1.06, with P=0.742 by chi-square test, which meant that no significant sex bias was existent, and that the offspring sex ratio of Tibetan antelope didn’t deviate from the theoretical value of 1︰1. The above-mentioned studies show that, there is no sex-biased selection during the female pregnancy period, or the selective effects may be too weak to affect the sex ratio. It is feasible to study the offspring sex ratio of Tibetan antelope by placenta samples, and this method could also be used for some other wild animals which have the similar reproductive model with Tibetan antelope.
引用本文:
陈家瑞, 张守栋, 李 邦, 张同作, 苏建平. 利用胎盘研究藏羚羊(Pantholops hodgsonii)初生性别比例[J]. 生命科学研究, 2016, 20(6): 471-474. CHEN Jia-Rui, ZHANG Shou-Dong, LI Bang, ZHANG Tong-Zuo, SU Jian-Ping. Studies on Offspring Sex Ratio of Tibetan Antelope (Pantholops hodgsonii) by Placenta. Life Science Research, 2016, 20(6): 471-474.
2016,Vol. 20
