Abstract:Abstract: Rapeseed is one of the most important oil crops in the world, and is also the main source of edible oil and forage protein in China. Here, seeds of Brassica napus L. pol cms maintainer line 2B were treated with 0.8% ethyl methane sulfonate (EMS) to construct mutant library. From the M2 generation,1 495 mutants were obtained, including leaf color, leaf type, early flowering, dwarf, male sterility, crack bud, petal shape and color, multi-branched and long-silique mutants etc, which accounted for 7.4% of the total M2 population. Then the agricultural traits of multi-branched and long-silique mutants were characterized. The results showed that the multi-branched mutant harbored 1.03 times more branches than the wild-type, and the yield per plant increased by 41.48%; the silique of the long-silique mutant was 1.69 times the length of that of the wild-type, the seed yield per plant increased by 49.66%, but there was no difference for the seed number per silique between the long-silique mutant and the wild-type. These results laid a foundation for further exploring the mutant-related genes and using these mutants in Brassica napus breeding.
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廖晓英, 阎晋东, 钟 鸣, 卓宇红, 吴 丹, 贺热情, 赵小英, 刘选明. 甘蓝型油菜EMS诱变及多分枝和长角突变体分析[J]. 生命科学研究, 2016, 20(5): 435-441. LIAO Xiao-Ying, YAN Jin-Dong, ZHONG Ming, ZHUO Yu-Hong, WU Dan, HE Re-Qing, ZHAO Xiao-Ying, LIU Xuan-Ming. EMS Mutagenesis and Analysis of Multi-branched and Long-silique Mutants in Brassica napus L.. Life Science Research, 2016, 20(5): 435-441.