Abstract：Abstract: The adenomatous polyposis coli (APC) gene has been discovered independently in a hereditary cancer syndrome termed familial adenomatous polyposis (FAP). Mutation of APC can lead to multiple neoplasms in mice, but the phenotype in a mouse with conditional knockout of the APC gene, which genetically removes APC from the intestinal simple epithelium, is not clear. Via Cre-LoxP recombination enzyme system, the APC gene was knocked out in the epithelial cells of the intestinal villi and crypt cells, and the phenotype was identified and analyzed. When VillinCre was crossed with APCfl/fl, VillinCre;APCfl/+ developed intestinal tumor spontaneously and Wnt signal pathway was activated in these neoplasms. Surprisingly, VillinCre;APCfl/fl never appeared by crossing VillinCre;APCfl/+ with APCfl/fl. The results showed that VillinCre; APCfl/+ mouse has been generated, which would be a wonderful model for further research of APC gene in intestine development and oncogenesis.
引用本文:
廖超男, 杨治平, 林良武, 杨智英, 蔡金杏, 熊 璐, 黄 河. 条件性敲除APC基因小鼠肠道腺瘤模型的构建[J]. 生命科学研究, 2016, 20(5): 424-428. LIAO Chao-Nan, YANG Zhi-Ping, LIN Liang-Wu, YANG Zhi-Ying, CAI Jin-Xing, XIONG Lu, HUANG He. Construction of Mouse Intestinal Adenoma Model via Conditionally Knocking Out APC from Epithelium. Life Science Research, 2016, 20(5): 424-428.
2016,Vol. 20
