Abstract：Abstract: Deferiprone could induce the expression of p53 and cause the apoptosis of cancer cells. RRM2B gene also plays an important role in the antiproliferation of cancer cells caused by iron chelators and is regulated by p53. To study the effects of deferiprone on cervical cancer cells and the relationship between p53 and RRM2B gene, the MTT assay was adopted for detecting the effects of deferiprone with different concentrations and durations on HeLa cell activity, and the flow cytometry was used for observing the apoptosis of HeLa cells. At the same time, the technologies of Western-blot and fluorogenic quantitative PCR were utilized for measuring the expression levels of p53 protein and RRM2B gene, respectively. The effect of p53 on the expression of RRM2B was further studied by using p53 inhibitor. The results showed that the activity of HeLa cells was decreased when increasing the concentration and duration of deferiprone treatment. The apoptosis was obvious when HeLa cells were treated with deferiprone at the concentration of 500 μmol/L for 48 h. Further studies showed that deferiprone significantly up-regulated the expression of p53 (P<0.05) and p53 significantly down-regulated the expression of RRM2B gene (P<0.05). Therefore, deferiprone could inhibit the proliferation of cervical cancer cells, which is associated with down-regulation of RRM2B expression by p53.
引用本文:
徐元屏, 刘玲华, 陈 莉, 李爱玲, 陈东祥. 去铁酮对宫颈癌HeLa细胞增殖和凋亡的影响[J]. 生命科学研究, 2016, 20(4): 290-295. XU Yuan-Ping, LIU Ling-Hua, CHEN Li, LI Ai-Ling, CHEN Dong-Xiang. Effects of Deferiprone on Proliferation and Apoptosis of HeLa. Life Science Research, 2016, 20(4): 290-295.
2016,Vol. 20
