Abstract：Abstract: Cytoprotective effects of ginsenoside Rg1 (GS-Rg1) against malondialdehyde (MDA)-suppressed osteogenic differentiation of mesenchymal stem cells (MSCs) and the possible mechanisms were investigated in vitro. After osteogenic differentiation of MSCs was induced by estrogenic medium, MSCs were pretreated with GS-Rg1 at different doses (10 mg/L，50 mg/L，100 mg/L) for 24 h and then treated with MDA (0.1 mmol/L) for 24 h. During the research, the formation of mineralized nodule and the activity of alkaline phosphatase (ALP) were examined. The mRNAs and protein expression of Runt-related transcription factor 2(Runx2) and ALP were analyzed by QRT-PCR and Western-blot, respectively. The results showed that pretreatment of GS-Rg1 increased calcium nodule formation and ALP activity, and up-regulated expression of ALP and Runx2, which indicated that GS-Rg1 protected MSCs from MDA-suppressed osteogenic differentiation． Therefore, the upregulation of ALP and Runx2 by GS-Rg1 may contribute to the protective role in osteogenic differentiation of MSCs.
引用本文:
黄思洋, 陈继尧, 王逸安, 张 君, 李 烨. 人参皂苷Rg1对丙二醛抑制小鼠间充质干细胞成骨分化的保护作用[J]. 生命科学研究, 2016, 20(2): 140-144. HUANG Si-Yang, CHEN Ji-Yao, WANG Yi-An, ZHANG Jun, LI Ye. Protective Effect of Ginsenoside Rg1 Against MDA-Suppressed Osteogenic Differentiation of Mesenchymal Stem Cells. Life Science Research, 2016, 20(2): 140-144.
2016,Vol. 20
