Abstract：Abstract: Latexin(Lxn) is a carboxypeptidase inhibitor in human and acts as a tumor-inhibiting factor. Through bioinformatic analysis, predictions on the promoter and CpG island of Lxn gene, as well as physicochemical properties, structural and functional characteristics of Lxn protein were performed. It was found that there are two promoters and two CpG islands in the gene and one CpG island is located in one promoter region. Lxn protein is composed of 222 amino acids, with isoelectric point of 5.52. It is a hydrophilic and unstable protein without a signal peptide and transmembrane domain. As for the advanced structure, four α-helices and 9 β-sheets were predicted in the secondary structure. The reliability of predicted tertiary structure reached up to 99.55% and the structure was proven to be stable by Ramachandran plot. The advanced structure influenced the function of protein. Lxn not only interacted with carboxypeptidase but also took part in inflammatory response and pain sense stimulated by temperature and so on. What′s more, the homologies of promoters and amino acids in primates were very high. The analysis results provide important messages for further studies on function of Lxn in human life activities.
引用本文:
王兆松, 赵文铖, 周 蒙, 魏 梅, 董秋萍, 许世磊. 人Latexin基因及蛋白质的生物信息学分析[J]. 生命科学研究, 2016, 20(2): 125-130. WANG Zhao-Song, ZHAO Wen-Cheng, ZHOU Meng, WEI Mei, DONG Qiu-Ping, XU Shi-Lei. Bioinformatic Analysis of Human Latexin Gene and Protein. Life Science Research, 2016, 20(2): 125-130.
2016,Vol. 20
