Abstract：Abstract: To explore the protective effect of different culture media on neonatal porcine islet cell clusters (NICCs) coated with stem cells. MSCs (mesenchymal stem cells, MSCs)-EPCs (endothelial progenitor cells, EPCs)-NICCs complex were cocultured in the NICC medium (Group A), the MSC medium (Group B), and the EPC medium (Group C), respectively. The secretory function and the gene expression level of NICCs, and the apoptosis rate of total cells was detected. The number of broken islet cells in group C was least, and the apoptosis rate was the least in group C. In addition the mRNA expression level of the insulin and glucagon gene were increased especially in group C (P<0.05). The EPC medium is the best medium for protecting NICC complex.
引用本文:
聂 唯, 马小倩, 刘 亮, 张 娟, 王 维. 不同培养基对干细胞-胰岛复合物培养后形态和功能的影响[J]. 生命科学研究, 2015, 19(6): 516-520. NIE Wei, MA Xiao-Qian, LIU Liang, ZHANG Juan, WANG Wei. Influence of Different Culture Media on the Morphology and Function of Stem Cells-Islet Complex. Life Science Research, 2015, 19(6): 516-520.
2015,Vol. 19
