Abstract：Abstract: To establish glucagon-like peptide-1 receptor (GLP-1R) cellular model for screening GLP-1R ligands. A eukaryotic expression vector pCMV6-GLP-1R-GFP was constructed and transfected into U2OS cells. A stable expressing GLP-1R-GFP U2OS cell line was established and the expression of GLP-1 receptor was confirmed by Western-blot with a GLP-1 specific antibody. The activities of expressed GLP-1R-GFP were investigated with the cellular redistribution of green fluorescence after stimulation with GLP-1 analogs. The sensitivity and specificity of this cell line was qualified and the results showed that the cell line was suitable for GLP-1 receptor ligand screening.
引用本文:
李明瑞, 季秀玲, 张 敏, 张宽仁, 刘东波, 魏云林. GFP荧光标记GLP-1受体细胞系的建立[J]. 生命科学研究, 2015, 19(6): 501-504. LI Ming-Rui, JI Xiu-Ling, ZHANG Min, ZHANG Kuan-Ren, LIU Dong-Bo, WEI Yun-Lin. Establishment of a GFP Fluorescence Labeled GLP-1 Receptor Cell Line. Life Science Research, 2015, 19(6): 501-504.
2015,Vol. 19
