Abstract：Abstract: Obtaining infectious clones often remains tedious, time-consuming and rather unpredictable, despite significant advances in molecular biology techniques during the last decades. In the case of Potyviruses, cDNA clones are frequently unstable due to the toxicity of some viral proteins for bacteria. The problem can be solved by inserting introns into the viral sequence, which requires additional steps in the cloning process and depends on the availability of suitable restriction sites in the viral sequence or adjudication of such sites by mutagenesis. For example with Papaya ringspot virus (PRSV), it will be described how to construct the infectious clones or other viral cDNA vectors without insert introns by using yeast homologous recombination system in two weeks.
引用本文:
赵光远, 庹德财, 沈文涛, 黎小瑛, 周 鹏. 利用酵母同源重组系统快速构建Potyvirus病毒侵染性克隆方法[J]. 生命科学研究, 2015, 19(6): 491-496. ZHAO Guang-Yuan, TUO De-Cai, SHEN Wen-Tao, LI Xiao-Ying, ZHOU Peng. A Simple and Rapid Method to Construct Potyvirus Infectious Clone by Yeast Homologous Recombination System. Life Science Research, 2015, 19(6): 491-496.
2015,Vol. 19
