Abstract：Abstract: 24 male Sprague dawley (SD) rats on a low-sodium diet were randomly divided into control group (olive oil, 1 mL/kg/d, i.p.), model group (CsA, 30 mg/kg/d, i.p.) and Klotho group (CsA, 30 mg/kg/d and Klotho 0.02 mg/kg, every other day, i.p.). Renal pathological changes were evaluated by Masson’s trichrome staining. Apoptosis was assessed by TUNEL assay and endoplasmic reticulum stress was estimated by detection of GRP78 and CHOP. Administration of CsA for 28 d caused renal lesions and increased the apoptosis, while Klotho treatment ameliorated these pathological changes. CsA treatment upregulated indicators of ER stress including GRP78 and CHOP, while Klotho prevented the increase in these indicators of ER stress. Collectively, Klotho therapy attenuates apoptosis via inhibiting ER stress in a rat model of chronic CsA-induced nephropathy.
引用本文:
李莎莎, 邓志勇, 何敖林, 叶建明, 刘其锋. Klotho对CsA慢性肾毒性大鼠肾脏内质网应激相关凋亡的影响[J]. 生命科学研究, 2015, 19(5): 422-425. LI Sha-Sha, DENG Zhi-Yong, HE Ao-Lin, YE Jian-Ming, LIU Qi-Feng. Klotho Ameliorates Cyclosporine-induced Chronic Nephrotoxicity Via Inhibition of Endoplasmic Reticulum Stress. Life Science Research, 2015, 19(5): 422-425.
2015,Vol. 19
