Abstract：Abstract: 100 SPF Kunming mice (Mus musculus) (20±2 g), half male and half female, were randomly divided into 5 groups, with 4 replications per group and 5 mice per replication. They were fed with diets that contained low and high dose of genetically modified (GM) rice Bar68-1,non-GM rice D68 or routine feed respectively for duration of 180 days. After 90 days, parental generation (P) bred the first filial generation (F1). Each generation was fed for 180 days respectively. On the 180th day, 5 mice of each group were sampled at random, and intestinal contents were collected for DNA isolation. The V3 region of 16S rDNA was amplified by PCR and analyzed via denaturing gradient gel electrophoresis (DGGE). Cs values for intergroup comparisons of samples did not show significant difference from each other (P>0.05), and some identical intestinal predominant flora was identified by sequence analysis of bands. The results showed that GM rice Bar68-1 had no significant effects on the microflora in Mus musculus intestine.
引用本文:
刘 金, 黄 毅, 孙艳波, 颜亨梅. 转Bar基因稻谷对小鼠（Mus musculus）肠道菌群的作用研究[J]. 生命科学研究, 2015, 19(5): 415-421. LIU Jin, HUANG Yi, SUN Yan-Bo, YAN Heng-Mei. Research on the Effects of Bar-transgenic Rice on the Microflora in Intestine of Mice (Mus musculus). Life Science Research, 2015, 19(5): 415-421.
2015,Vol. 19
