Abstract：Abstract: The development of short-interfering RNA (siRNA) has provided great hope for therapeutic targeting of specific genes responsible for pathological disorders. However, the poor cellular uptake of siRNA together with the low permeability of the cell membrane to negatively charged molecules, remain major obstacles to clinical development. So far there is no universal method for siRNA delivery as they all present some limitations. Some strategies have been proposed to improve the delivery of synthetic siRNAs in both cultured cells and in vivo. Cell-penetrating peptides (CPPs) constitute very promising tools for non-invasive cellular import of siRNA and non-covalent CPPs-based strategies have been successfully applied for in vitro and in vivo delivery of therapeutic siRNA molecules. A new peptide-based system, CADY, for efficient delivery of siRNA in both primary and suspension cell lines has been described recently. CADY is a secondary amphipathic peptide able to form stable non-covalent complexes with siRNA and to improve their adherent and suspension cell lines uptake, which shows high potential in clinical use.
引用本文:
杨成良, 王 君, 邹黎黎. 以两亲性细胞膜穿透肽为基础的siRNA递送策略[J]. 生命科学研究, 2015, 19(4): 353-356. YANG Cheng-Liang, WANG Jun, ZOU Li-Li. An Amphipathic Cell-penetrating Peptide-Based Strategy for siRNA Delivery. Life Science Research, 2015, 19(4): 353-356.
2015,Vol. 19
