Abstract：Abstract: To investigate the effects of quercetin on human chondrosarcoma cell migration, invasion and epithelial interstitial transformation (EMT) and the regulatory role of Janus kinase 2/signal transduction and transcription promoter 3 (JAK2/STAT3) signaling pathway, human chondrosarcoma SW-1353 cells were cultured in vitro and treated with quercetin of different concentrations for 24 h. The optimal concentration of quercetin (50.0 μmol/L) was selected by cell counting kit (CCK-8) for subsequent experiments. The cells were divided into control group with no intervention, quercetin group with 50.0 μmol/L quercetin, positive control group with 6.0 μmol/L adriamycin, inhibitor group involving 50.0 μmol/L quercetin +50.0 μmol/L JAK2/STAT3 pathway inhibitor AG490, and activator group involving 50.0 μmol/L quercetin +0.5 μmol/L JAK2/STAT3 pathway activator colivelin. After 24 h of intervention, cell morphology, migration, invasion, and expression levels of JAK2/STAT3 pathway and EMT-related proteins were analyzed by inverted microscope, Transwell chamber and Western blot. The experimental results showed that the optimum concentration of quercetin was determined by CCK-8 method to be 50.0 μmol/L. Compared with the control group, the growth of quercetin group were positive control group were inhibited, the cell migration number, invasion number, expression levels of N-cadherin, Vimentin, fibronectin (FN), p-JAK2 and p-STAT3 in quercetin group and positive control group decreased (P<0.05), E-cadherin expression level increased (P<0.05); compared with quercetin group, the change trend of the above indexes in inhibitor group was more significant (P<0.05), while the change of these indexes in activator group was reversed (P<0.05). Quercetin can inhibit the growth, migration, invasion and EMT progression of human chondrosarcoma SW-1353 cells, possibly by blocking the JAK2/STAT3 signaling pathway. Key words: chondrosarcoma; quercetin; JAK2/STAT3 signaling pathway; migration; invasion （Acta Laser Biology Sinica, 2023, 32(3): 240-246）
引用本文:
李 毅，张 恒，赵维彪. 槲皮素阻滞JAK2/STAT3信号通路对软骨肉瘤细胞迁移、侵袭和EMT的抑制作用研究[J]. 激光生物学报, 2023, 32(3): 240-246. LI Yi, ZHANG Heng, ZHAO Weibiao. Effect of Quercetin on Chondrosarcoma Cell Migration, Invasion and EMT by Blocking JAK2/STAT3 Signaling Pathway. journal1, 2023, 32(3): 240-246.
